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Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

Overview
148.00 GBP valid until 2025-06-21
Use promo code "24112" to get your promotional price.

Code: 50

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.
Includes:

50 Reaction Kit: 1 x 50μL of 20X Enzyme mix (MuLV and RNase inhibitor protein); 1 x 500μL of 2X RT Buffer Mix (includes dNTPs, random octamers, and oligo dT-16)

Product Code. 10704217

Quantity Price
1 £ 222.0 / Each
In stock, ready to ship: 94
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For Research Use Only. All usage must comply with product instructions.
 

  • Description and Specification
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Description and Specification

Specification

Content And Storage

• 20X Enzyme mix (1 x 50 μL)
• 2X RT Buffer Mix (1 x 500 μL)

Store at –15 to –25°C.
Buffer Mix may be stored at 2–8°C for up to 6 months.
Format Kit
Reaction Speed 30 to 60 min.
Technique Reverse Transcription
Optimal Reaction Temperature 37°C
Quantity 50 reactions
Reverse Transcriptase MultiScribe
Ribonuclease H Activity Yes
Shipping Condition Dry Ice
For Use With (Application) Real Time PCR (qPCR),RT-PCR
For Use With (Application) Real Time PCR (qPCR),RT-PCR
Final Product Type First-Strand cDNA
No. of Reactions 50 Reactions
Reaction Format Premixed Components
Reagent Type Reverse Transcription
Size (Final Product) Up to 7 kb
Starting Material RNA

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts.

Features of the High Capacity RNA-to-cDNA Kit include:
• Fast reaction time (typically 30–60 min)
• Convenient and simple workflow with few pipetting steps
• Reliable reverse transcription of both abundant and limited targets
• Optimized two-step protocol enables multiple PCR reactions from a single RT reaction

Streamline cDNA synthesis while maintaining detection sensitivity
Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

Order Info: Shipping Condition: Dry ice

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