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Invitrogen™ Mouse Leptin ELISA Kit

ELISA

Overview
396.72 GBP valid until 2025-12-31
Use promo code "24080" to get your promotional price.

Code: 50

Additional Details:
Additional Details: Weight: 0.50000kg



Product Code. 10338283

Quantity Price
1 £ 522.0 / Each
Estimated Shipment
04-08-2025
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

Accession Number P41160
Assay Range 93.8 to 6000 pg/mL
Assay Sensitivity <50 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma,Serum,Supernatant
Sample Type Plasma,Serum,Supernatant
Sample Type Plasma,Serum,Supernatant
Target Species Mouse
For Use With (Application) Colorimetric Detection
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 16846
Gene Symbol ob,obese
Incubation Time 4 hr.
Interassay CV 6.1%
Intraassay CV 5.2
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Kit Contents Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Label or Dye HRP
Marker Gene Leptin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 20 μL, Serum 20 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Target Leptin
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs

Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

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