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Thermo Scientific™ Pierce™ BCA Protein Assay Reagent A

Estimate visually, or measure with a standard spectrophotometer or plate reader at 562 nm.

£96.25 - £618.0

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Specification

Content And Storage Store at room temperature.
Description Pierce BCA Protein Assay Reagent A
Assay BCA Assay
Detection Method Colorimetric
Product Line Pierce™
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Disclaimers: For Research Use Only. Not for use in diagnostic procedures.


Specification

Content And Storage Store at room temperature.
Description Pierce BCA Protein Assay Reagent A
Assay BCA Assay
Detection Method Colorimetric
Product Line Pierce™
Product Type Protein Quantitation Assay
Specificity Not Target-Specific
For Use With (Application) Solution-based Detection, Absorbance
For Use With (Equipment) Spectrophotometer, Microplate Reader

  • A component of the Pierce BCA Protein Assay Kit
  • Kit is a two-component, high-precision, detergent-compatible assay that is used for total protein concentration determination compared to a protein standard
  • Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research
  • The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications
  • Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy
  • Estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
  • Exhibits less protein-to-protein variation than dye-binding methods (Bradford)
  • Unaffected by typical concentrations of most ionic and non-ionic detergents
  • Assay has a 30 min. incubation; much easier and four times faster than classical Lowry methods
  • Linear working range for BSA of 20 to 2000 μg/mL
  • Detect down to 5 μg/mL with the enhanced protocol
  • Store at room temperature

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