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Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

Overview
276.45 GBP valid until 2025-12-31
Use promo code "24357" to get your promotional price.

Code: NEW

Additional Details:
Additional Details: Weight: 0.00100kg



Disclaimers: For Research Use Only.
Includes: 4x1.25 mL Platinum II PCR Master Mix (2X), 2x1.25 mL Platinum GC Enhancer, 4x1.25 mL Water, nuclease-free

Product Code. 15899844

Quantity Price
1 £ 311.0 / 200 reactions
In stock, ready to ship: 5
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  • Description and Specification
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Description and Specification

Specification

Concentration 2X
Content And Storage • Platinum II PCR Master Mix (2X), 4 x 1.25 mL
• Platinum GC Enhancer, 2 x 1.25 mL
• Nuclease-free water, 4 x 1.25 mL

Store at -20°C in a non-frost-free freezer.
Format Tube
GC-Rich PCR Performance High
Polymerase Platinum II Taq Hot-Start DNA Polymerase
Reaction Speed Fast or Standard
Product Type Hot Start PCR Master Mix
Quantity 200 reactions
Shipping Condition Dry Ice
For Use With (Application) Hot-start PCR,Hot-start PCR
For Use With (Application) Hot-start PCR,Hot-start PCR
Fidelity (vs. Taq) 1X
Hot Start Built-In Hot Start
No. of Reactions 200 Reactions
Overhang 3'-A
Reaction Format SuperMix or Master Mix
Size (Final Product) 5 kb or less
Starting Material DNA
Color Colorless

Platinum II Hot-Start Green PCR Master Mix also includes two tracking dyes for direct loading of PCR products on gels.

Features

  • Innovative buffer enables universal annealing temperature by isostabilizing primer-template duplex structures
  • Engineered Taq DNA polymerase confers fast cycling and resistance to common inhibitors
  • Hot-start technology enables superior specificity, sensitivity, and yields and allows for room temperature reaction setup
  • Green PCR buffer reduces pipetting errors with direct gel loading

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • Amplification and improved yields of GC-rich targets
  • RT-PCR
  • Genotyping
  • High-throughput PCR

Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq DNA polymerase that shows increased resistance to reaction inhibitors originating from sample material or DNA purification steps. The polymerase has a higher DNA synthesis rate and delivers PCR results more than two times faster than other Taq DNA polymerases. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C. This automatic hot start provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. With Platinum II Hot-Start PCR Master Mix (2X), different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified.

Notes

  • For applications that require PCR product analysis by absorbance or fluorescence excitation, colorless format is recommended.

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