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  6. Macherey-Nagel™ Protino™ Ni-NTA Agarose

Macherey-Nagel™ Protino™ Ni-NTA Agarose

Method of choice for His-tag protein purification with best performance.

388.00 CHF - 6185.00 CHF

Spezifikation

Bindungsvermögen < 50 mg/ml abgesetzte Agarose
Beschreibung Wässrige Suspension
Inhalt und Lagerung 4°bis 8 °C
Zur Verwendung mit (Anwendung) Aufreinigung von Polyhistidin-getaggten Proteinen
Ziel Protein
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Macherey-Nagel™
745400.25 		25 ml
388.00 CHF
25 Milliliter
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Macherey-Nagel™
745400.500 		500 ml
6185.00 CHF
500 Milliliter
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Verfügbar ab: 10-07-2025
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Description

Description

  • Enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
  • Proteins from any expression system can be purified under native or denaturing conditions.
  • Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions.
  • Consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6% cross-linked agarose beads that are suitable for batch binding, gravity flow, and FPLC™ columns.
  • The resin is precharged with Ni2+ ions and therefore ready to use.
  • Technology: Immobilized metal ion affinity chromatography
  • Batch binding, gravity flow column chromatography, MPLC / FPLC applications
  • Matrix: 6 % beaded agarose (cross-linked), precharged with Ni2+
  • Bead size: 45 to 165 μm
Specifications

Specifications

< 50 mg/ml abgesetzte Agarose
4°bis 8 °C
Protein
NTA (Nitrilotriessigsäure)
Wässrige Suspension
Aufreinigung von Polyhistidin-getaggten Proteinen
Agarose
Videos
Safety and Handling

Safety and Handling

Signal Word
  • Achtung

Hazard Category
  • Entzündbare Flüssigkeit Kategorie 3
  • Sensibilisierung der Haut Kategorie 1

Hazard Statement
  • H226-Flüssigkeit und Dampf entzündbar.
  • H317-Kann allergische Hautreaktionen verursachen.

Precautionary Statement
  • P210-Von Hitze, heißen Oberflächen, Funken, offenen Flammen und anderen ndquellen fernhalten. Nicht rauchen.
  • P261-Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermei den.
  • P280-Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz tragen.

Supplemental information
  • MIXTURE LIST-Enthält: ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU), resin suspension with Ni in

SDS
Documents

Documents

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