R&D Systems™ Recombinant Human TGF-beta 1 Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Overview
676.00 GBP valid until 2025-07-30
Use promo code "24078" to get your promotional price.



Additional Details:
Additional Details: Weight: 0.00970kg



Product Code. 18126890

Quantity Price
1 £ 845.0 / 10µg
In stock, ready to ship: 117
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  • Description and Specification
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Description and Specification

Specification

Accession Number P01137
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in acetonitrile and TFA with BSA as a carrier protein.
Gene ID (Entrez) 7040
Molecular Weight (g/mol) M.W. (Observed): 12 kDa, reducing conditions 24 kDa, non-reducing conditions; M.W. (theoretical): 12.8 kDa (monomer)
Name TGF-beta 1
Quantity 10 μg
Source Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein Ala279-Ser390
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Structural Form Disulfide-linked homodimer
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias CEDLAP, DPD1, latency-associated peptide, TGF beta1, TGFB, TGFB1, TGFbeta, TGFbeta 1, TGF-beta 1 protein, TGF-beta-1, transforming growth factor beta-1, transforming growth factor, beta 1
Gene Symbol TGFB1
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Purified recombinant human TGF-beta 1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, reconstitute 2 μg vials at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin. Reconstitute 10 μg or larger vials at 100 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin.
Purity or Quality Grade >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein TGF-beta 1

Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.

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