Invitrogen™ SuperScript™ IV VILO™ Master Mix

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

Overview
498.40 GBP valid until 2025-09-30
Use promo code "24076" to get your promotional price.



Additional Details:
Additional Details: Weight: 0.50000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15523145

Quantity Price
1 £ 712.0 / 50 reactions
In stock, ready to ship: 36
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  • Description and Specification
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Description and Specification

Specification

Concentration 5X
Content And Storage

• SuperScript IV VILO Master Mix (200 μL)
• SuperScript IV VILO Master Mix 'No RT' Control (200 μL)
• ezDNase Enzyme (50 μL)
• 10X ezDNase Buffer (100 μL)
• Nuclease-free water (1.25 mL)

Store at -15°C to -25°C.

Detection Method qPCR
Format Master Mix
GC-Rich PCR Performance High
Reaction Speed 10 min.
Technique Reverse Transcription
Optimal Reaction Temperature 50°C
Quantity Each
Reverse Transcriptase SuperScript IV
Ribonuclease H Activity Reduced
Shipping Condition Dry Ice
For Use With (Application) Real Time PCR (qPCR)
Final Product Type First-Strand cDNA
No. of Reactions 50 Reactions
Reaction Format Master Mix
Reagent Type Reverse Transcription
Size (Final Product) Up to 10 kb
Starting Material RNA
Includes Kit with ezDnase

SuperScript IV VILO Master Mix features include:
• Fast RT reactions in just 10 minutes
• Convenient one-tube cDNA reaction master mix for two-step RT-qPCR
• High yields reduce Ct values by more than two cycles ahead of all other reverse transcription reagents
• Efficient reaction even with low template amounts and suboptimal purity samples
• gDNA removal in as few as two minutes (SSIV VILO Master Mix with ezDNase only)

SSIV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV Reverse Transcriptase and further optimized buffer. These components enable efficient cDNA synthesis at higher temperatures and in less time. SSIV VILO master mix provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates. It is your new tool for more efficient and reproducible RT-qPCR.

SuperScript IV VILO Master Mix with ezDNase is available for better removal of genomic DNA and to further accelerate the RT-qPCR workflow. The extremely simplified genomic DNA removal step dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment.

SSIV VILO master mix results in more cDNA in less time with less pipetting, less variation, less reaction inhibition, less gDNA interference, and with less sample than other cDNA synthesis kits or master mixes for RT-qPCR. Additional ezDNase may also be purchased separately.

Source
Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance, and to reduce RNase H activity

Performance and quality testing
Assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product

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